Journal:

Article Title: Global Expression Profiling and Physiological Characterization of Corynebacterium glutamicum Grown in the Presence of l -Valine

doi: 10.1128/AEM.69.5.2521-2532.2003

Figure Lengend Snippet: ORFs showing altered relative mRNA levels in response to l -valine in the valine production strain VAL1 a

Article Snippet: Proteins showing altered abundances on 2-D gels in response to l -valine in the wild-type C. glutamicum ATCC 13032 table ft1 table-wrap mode="anchored" t5 TABLE 5. caption a7 ORF NCBI Annotation Gene Intensity b −Val +Val Ratio 1504 NCgl0899 Similarity to 2-nitropropane dioxygenase ( Williopsis mrakii ) 0.4 0.2 0.5 3194 NCgl2167 Pyruvate dehydrogenase component E1 aceE ( C. glutamicum ) 0.6 0.2 0.3 990 NCgl0478 Strong similarity to translation elongation factor EF-G fusA ( E. coli ) 0.3 0.6 2.3 1414 NCgl0827 Strong similarity to PurH bifunctional enzyme ( E. coli ) 0.3 1.5 4.3 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 1.6 4.9 3.1 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.8 3.1 3.6 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.4 1.5 4.1 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.2 0.7 4.2 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.5 2.4 5.1 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.6 3.4 5.3 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.2 1.0 5.9 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.2 1.6 7.2 Open in a separate window a Abbreviations, conditions, and statistical analysis are described in footnotes to Table . b +Val, growth under addition of 40 mM l -valine.

Techniques:

Journal:

Article Title: Global Expression Profiling and Physiological Characterization of Corynebacterium glutamicum Grown in the Presence of l -Valine

doi: 10.1128/AEM.69.5.2521-2532.2003

Figure Lengend Snippet: Proteins showing altered abundances on 2-D gels in response to l -valine in the valine production strain VAL1 a

Article Snippet: Proteins showing altered abundances on 2-D gels in response to l -valine in the wild-type C. glutamicum ATCC 13032 table ft1 table-wrap mode="anchored" t5 TABLE 5. caption a7 ORF NCBI Annotation Gene Intensity b −Val +Val Ratio 1504 NCgl0899 Similarity to 2-nitropropane dioxygenase ( Williopsis mrakii ) 0.4 0.2 0.5 3194 NCgl2167 Pyruvate dehydrogenase component E1 aceE ( C. glutamicum ) 0.6 0.2 0.3 990 NCgl0478 Strong similarity to translation elongation factor EF-G fusA ( E. coli ) 0.3 0.6 2.3 1414 NCgl0827 Strong similarity to PurH bifunctional enzyme ( E. coli ) 0.3 1.5 4.3 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 1.6 4.9 3.1 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.8 3.1 3.6 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.4 1.5 4.1 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.2 0.7 4.2 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.5 2.4 5.1 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.6 3.4 5.3 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.2 1.0 5.9 2793 NCgl1222 Acetolactate synthase large-chain ilvB ( C. glutamicum ) ilvB 0.2 1.6 7.2 Open in a separate window a Abbreviations, conditions, and statistical analysis are described in footnotes to Table . b +Val, growth under addition of 40 mM l -valine.

Techniques:

Journal: BMC Cancer

Article Title: RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer

doi: 10.1186/1471-2407-2-28

Figure Lengend Snippet: Confirmation of specificity of interactions observed in yeast 2-hybrid assay. Panels show yeast transformed with a) psoriasin and RanBPM expression plasmids (left) and RanBPM alone (right), and yeast transformed with b) Psoriasin alone, c) psoriasin and empty prey vector, and d) psoriasin with a control gene not isolated in the primary screen. In panels b-d, plates on left are Histidine + (control) and plates on the right are Histidine – (test). Panel a) shows activation of LacZ reporter gene only occurs in yeast transformed with both expression plasmids (left) and no activation in yeast transformed with RanBPM alone (right). Panel b) shows that psoriasin alone cannot activate the Histidine reporter gene as demonstrated by absence of growth on Histidine – plate. Panels c) and d) show that there is a specific interaction necessary for activation of the reporter gene.

Article Snippet: Psoriasin, cyclophilin 33 and RanBPM PCR products were each cloned into pGEM-T Easy (Promega), sequenced and then serially diluted separately to construct the standard curve used for quantification of mRNA expression.

Techniques: Y2H Assay, Transformation Assay, Expressing, Plasmid Preparation, Isolation, Activation Assay

Journal: BMC Cancer

Article Title: RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer

doi: 10.1186/1471-2407-2-28

Figure Lengend Snippet: In vitro interaction of psoriasin and RanBPM as determined by co-immunoprecipitation. 35 S-Met labelled proteins were generated. Psoriasin (lane 1) and RanBPM (lane 2) were electrophoresed through a denaturing polyacrylamide gel and detected by autoradiography. Psoriasin binds to and co-immunoprecipitates with RanBPM (lane 4). RanBPM and psoriasin do not bind to protein G-beads on their own, (lane 3 and 5 respectively). RanBPM does not interact with mouse submaxillary gland protein when either RanBPM (lane 6) or mouse submaxillary gland protein (lane 7) is precipitated.

Article Snippet: Psoriasin, cyclophilin 33 and RanBPM PCR products were each cloned into pGEM-T Easy (Promega), sequenced and then serially diluted separately to construct the standard curve used for quantification of mRNA expression.

Techniques: In Vitro, Immunoprecipitation, Generated, Autoradiography

Journal: BMC Cancer

Article Title: RanBPM interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in breast cancer

doi: 10.1186/1471-2407-2-28

Figure Lengend Snippet: Relationship Between RanBPM and Psoriasin Expression and Clinical-Pathological Parameters in Human Breast Cancer

Article Snippet: Psoriasin, cyclophilin 33 and RanBPM PCR products were each cloned into pGEM-T Easy (Promega), sequenced and then serially diluted separately to construct the standard curve used for quantification of mRNA expression.

Techniques: Expressing